BIOSENSORS eguqukayo RT-IC Ubaleko Buffer
Ingcaciso yeMveliso
Inombolo yoMyalelo: BU-RB-10-1
Itheyibhile 1. Imixholo kunye noLwazi lokuGcina
| Izinto eziphathekayo | Ukugxila | Isixa | Ugcino |
| I-RT-IC Running Buffer (RB 1) (1.37 M NaCl,
26.7 mM KCl, 14.7 mM KH2PO4, 81 mm Na2HPO4, 0.1% I-Pluronic, ngaphandle kwe-calcium, ngaphandle kwe-magnesium; 0.2 µm icwecwe elinyumba) |
10 x isitokhwe | 50 ml | 2-8 °C |
Isetyenziselwa uphando kuphela.
Le mveliso inobomi obulinganiselweyo beshelufu, nceda ubone umhla wokuphelelwa kwileyibhile.
Ukulungiselela
Nciphisa isisombululo esipheleleyo se-50 mL 10x RT-IC Running Buffer 1 ngokuxuba kunye ne-450 mL yamanzi e-ultrapure. Emva kwe-dilution RT-IC Running Buffer ikulungele ukusetyenziswa (137 mM NaCl, 2.67 mM KCl, 1.47 mM KH2PO4, 8.1 mM Na2HPO4, 0.01 % Pluronic, ngaphandle kwe-calcium, ngaphandle kwe-magnesium).
Isithinteli esihlanjululweyo kufuneka sigcinwe kwi-2-8 ° C.
Isihluzi esisebenzayo ngaphambi kokusetyenziswa kwi-heliXcyto.
Qhagamshelana
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Jemani
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BIOSENSORS eguqukayo RT-IC Ubaleko Buffer [pdf] Incwadi yokusebenzisa BU-RB-10-1, RT-IC Running Buffer, RT-IC, Running Buffer, Buffer |