BIOSENSORS eguquguqukayo AS-2-Rc Ngokupheleleyo iAutomated Laboratory Analysis System

Iinkcukacha

• Igama lemveliso: heliX + ADAPTER STRAND 2 ngedayi ebomvu Rc
• Umenziumthombo: Dynamic Biosensors GmbH & Inc.
• Umzekelo: AS-2-Rc v5.1
• Ezona mpawu:
  • Amabala ama-2 anee-2 ezahlukeneyo zokulandelelana kweankile kwi-DNA-encoded addressing.
• Inombolo yoku-oda: AS-2-Rc
• Ugxininiso: 400 nM
• Ugcino: Umnqwazi omhlophe, nceda ubone umhla wokuphelelwa kwileyibhile. Gwema imijikelo emininzi yokunyibilikisa ngokuyicaphula i-nanolever.

Imiyalelo yokusetyenziswa kwemveliso

Ukulungiselela | MIX&RUN

1. Hlanganisa i-Adapter strand 1 - Rc (400 nM) kunye ne-conjugated Ligand strand kunye ne-ligand 1 (500 nM) kwi-1: 1 ratio (v / v).
2. Hlanganisa i-Adapter strand 2 - Rc (400 nM) kunye ne-conjugated Ligand strand kunye ne-ligand 2 (500 nM) kwi-1: 1 ratio (v / v).
3. Faka ngokuhlukileyo izisombululo ezibini zesinyathelo 1 kunye ne-2 kwindawo yokushisa kwegumbi kwi-600 rpm imizuzu engama-30 ukuqinisekisa ukuxutywa okupheleleyo.
4. Hlanganisa izisombululo zenyathelo 1 kunye ne-2 kwi-1: umlinganiselo we-1 (v / v). Isisombululo silungele ukusebenza kwe-biochip.

Ezona mpawu

• I-Adapter strand 2 yokusebenza kwe-heliX® Adapter Chip Spot 2.
• Iyahambelana ne-heliX® Adapter Chip.
• Kubandakanya imicu ye-Adapter ye-50 yokuvuselela.
• Ilungele uMIX&RUN sample yokulungiselela.
• I-Adapter strand 2 ithwala idayi ebomvu ye-hydrophobic (Rc) kunye nentlawulo engathathi hlangothi.

heliX® Adapter Chip Overview

Amabala ama-2 anee-2 ezahlukeneyo zokulandelelana kweankile kwi-DNA-encoded addressing.

Ingcaciso yeMveliso

Umyalelo Inani: AS-2-Rc

Itheyibhile 1. Imixholo kunye noLwazi lokuGcina

• Isetyenziselwa uphando kuphela.
• Le mveliso inobomi obulinganiselweyo beshelufu, nceda ubone umhla wokuphelelwa kwileyibhile.
• Ukunqanda imijikelo emininzi yokunyibilika komkhenkce nceda uliquot the nanolever

Ukulungiselela | MIX&RUN

Ukuhlanganiswa kwesisombululo kwi-adapter kunye ne-ligand strands:

1. Hlanganisa i-Adapter strand 1 - Rc (400 nM) kunye ne-conjugated Ligand strand kunye ne-ligand 1 (500 nM) kwi-1: 1 ratio (v / v).
2. Hlanganisa i-Adapter strand 2 - Rc (400 nM) kunye ne-conjugated Ligand strand kunye ne-ligand 2 (500 nM) kwi-1: 1 ratio (v / v).
3. Fukamisa ngokwahlukileyo izisombululo ezibini zenyathelo 1 kunye ne-2 kwi-RT kwi-600 rpm imizuzu engama-30 ukuqinisekisa ukuxutywa okupheleleyo.
4. Hlanganisa isisombululo sesinyathelo 1 kunye ne-2 kwi-1: umlinganiselo we-1 (v / v). Isisombululo sikulungele ukusetyenziswa kwi-biochip functionalization. Ukuzinza kwesisombululo kuhambelana nokuzinza kwee-molecule ze-ligand.

Itheyibhile 2. Izinto ezongezelelweyo zokusebenza kwendawo 1 kunye nendawo yesalathiso 2.

Example
Umthamo ofunekayo we-3 yokusebenza: 100 μL kunye noxinzelelo lokugqibela lwe-100 nM.

Emva kwexesha lokufukamela, xuba ibhodlela yoku-1 kunye ne-2 ukuze ufumane i-100 μL yesisombululo se-DNA esele sikulungele ukusetyenziswa.

Qhagamshelana

Dynamic Biosensors GmbH Perchtinger Str. 8/10 81379 Munich Germany

Dynamic Biosensors, Inc.
300 Trade Centre, Suite 1400 Woburn, MA 01801 USA

• Ulwazi lweodolo order@dynamic-biosensors.com
• Uxhaso lobuchwepheshe support@dynamic-biosensors.com
• www.dynamic-biosensors.com

Izixhobo kunye neetshiphusi zenziwe ngobunjineli kwaye zenziwe eJamani.

©2024 Dynamic Biosensors GmbH | Dynamic Biosensors, Inc. Onke amalungelo agciniwe.

1. TE40: 10 mM Tris, 40 mM NaCl, 0.05 % Tween20, 50 μM EDTA, 50 μM EGTA
2. Ukuba iprotheni ayizinzile kwi-PE40 (TE40, HE40), nceda ujonge ukuhambelana kwe-buffer kunye neshiti lokuhambelana ne-switchSENSE®.

www.dynamic-biosensors.com

FAQ

Umbuzo: Buthini ubomi beshelufu yemveliso?
A: Imveliso inobomi obunqamlekileyo beshelufu, nceda ubhekisele kumhla wokuphelelwa kwileyibhile.

Umbuzo: Ndifanele ndiyigcine njani imveliso ukugcina uzinzo?
A: Gcina imveliso ngekepusi emhlophe ukuphepha ukuvezwa ekukhanyeni. Gwema imijikelo yokunyibilika komkhenkce ngokucaphula i-nanolever.

Umbuzo: Ndifanele ndilungiselele njani isisombululo se-biochip functionalization?
A: Landela amanyathelo okulungiselela i-MIX&RUN anikezelwe kwincwadi yomsebenzisi ukulungiselela isisombululo se-biochip functionalization.

Amaxwebhu / Izibonelelo

BIOSENSORS eguquguqukayo AS-2-Rc Ngokupheleleyo iAutomated Laboratory Analysis System [pdf] Incwadi yokusebenzisa I-AS-2-Rc, i-AS-2-Rc iNkqubo yokuHlalutya iLabhoratri ngokuPheleleyo, iSixokelelwano sokuHlalutya iLebhu yeLabhoratri, iNkqubo yokuHlalutya iLabhoratri, iNkqubo yokuHlalutya iLabhoratri, iNkqubo yokuHlalutya, iNkqubo

Iimbekiselo

• Incwadi yokusebenzisa